Radiative Transfer Effects in He I Emission Lines

We consider the effect of optical depth of the 2 ^{3}S level on the nebular recombination spectrum of He I for a spherically symmetric nebula with no systematic velocity gradients. These calculations, using many improvements in atomic data, can be used in place of the earlier calculations of Robbins. We give representative Case B line fluxes for UV, optical, and IR emission lines over a range of physical conditions: T=5000-20000 K, n_{e}=1-10^{8} cm^{-3}, and tau_{3889}=0-100. A FORTRAN program for calculating emissivities for all lines arising from quantum levels with n < 11 is also available from the authors. We present a special set of fitting formulae for the physical conditions relevant to low metallicity extragalactic H II regions: T=12,000-20,000 K, n_{e}=1-300 cm^{-3}, and tau_{3889} < 2.0. For this range of physical conditions, the Case B line fluxes of the bright optical lines 4471 A, 5876 A, and 6678 A, are changed less than 1%, in agreement with previous studies. However, the 7065 A corrections are much smaller than those calculated by Izotov & Thuan based on the earlier calculations by Robbins. This means that the 7065 A line is a better density diagnostic than previously thought. Two corrections to the fitting functions calculated in our previous work are also given.Comment: To be published in 10 April 2002 ApJ; relevant code available at ftp://wisp.physics.wisc.edu/pub/benjamin/Heliu

Theoretical He I Emissivities in the Case B Approximation

We calculate the He I case B recombination cascade spectrum using improved radiative and collisional data. We present new emissivities over a range of electron temperatures and densities. The differences between our results and the current standard are large enough to have a significant effect not only on the interpretation of observed spectra of a wide variety of objects but also on determinations of the primordial helium abundance.Comment: Accepted to ApJ

Contemporary Innovation Policy and Instruments: Challenges and Implications

In this paper we review major theoretical (neoclassical economics, evolutionary, systemic and knowledge-based) insights about innovation and we analyse their implications for the characteristics of contemporary innovation policy and instruments. We show that the perspectives complement each other but altogether reveal the need to redefine the current general philosophy as well as the modes of operationalisation of contemporary innovation policy. We argue that systemic instruments ensuring proper organisation of innovation systems give a promise of increased rates and desired (more sustainable) direction of innovation.systemic instruments, innovation policy, innovation theory, policy mix, innovation system, sustainability

Understanding the build-up of a Technological Innovation System around Hydrogen and Fuel Cell Technologies

This study provides insight into the development of hydrogen and fuel cell technologies in the Netherlands (1980-2007). This is done by applying a Technological Innovation System (TIS) approach. This approach takes the perspective that a technology is shaped by a surrounding network of actors, institutions and technologies. When a technology is in an early stage of development, a TIS has yet to be built up in order to propel technological progress. This paper focuses on the historical build-up of the hydrogen and fuel cell innovation system in the Netherlands. The research focuses on processes that accelerated or slowed down the developments of hydrogen and fuel cell technologies. We suggest that this framework is helpful for actors who intend to accelerate the development and deployment of hydrogen and fuel cells in other countries.fuel cell; technological innovation system; system functions; cumulative causation.

The Primordial Abundance of He4: An Update

We include new data in an updated analysis of helium in low metallicity extragalactic HII regions with the goal of deriving the primordial abundance of He4 (Y_P). We show that the new observations of Izotov et al (ITL) are consistent with previous data. However they should not be taken in isolation to determine (Y_P) due to the lack of sufficiently low metallicity points. We use the extant data in a semi-empirical approach to bounding the size of possible systematic uncertainties in the determination of (Y_P). Our best estimate for the primordial abundance of He4 assuming a linear relation between He4 and O/H is Y_P = 0.230 \pm 0.003 (stat) based on the subset of HII regions with the lowest metallicity; for our full data set we find Y_P = 0.234 \pm 0.002 (stat). Both values are entirely consistent with our previous results. We discuss the implications of these values for standard big bang nucleosynthesis (SBBN), particularly in the context of recent measurements of deuterium in high redshift, low metallicity QSO absorption-line systems.Comment: 26 pages, latex, 6 ps figure

Understanding the formative stage of Technological Innovation System development. The case of natural gas as an automotive fuel

This study contributes to insights into mechanisms that influence the successes and failures of emerging energy technologies. It is assumed that for an emerging technology to fruitfully develop, it should be fostered by a Technological Innovation System (TIS), which is the network of actors, institutions and technologies in which it is embedded. For an emerging technology a TIS has yet to be built up. The research focuses on the dynamics of this build-up process by mapping the development of seven key activities: so-called system functions. The main contribution revolves around the notion of cumulative causation, or the phenomenon that the build-up of a TIS may accelerate due to system functions reinforcing each other over time. As an empirical basis, an analysis is provided of the historical development of the TIS around automotive natural gas technology in the Netherlands (1970-2007). The results show that this TIS undergoes a gradual build-up in the 1970s, followed by a breakdown in the 1980s and, again, a build-up from 2000-2007. It is shown that, underlying these trends, there are different forms of cumulative causation, here called motors of innovation. The study provides strategic insights for practitioners that aspire to support such motors of innovation.functions of technological innovation systems; cumulative causation; automotive natural gas.

How to change the oligomeric state of a circular protein assembly: switch from 11-subunit to 12-subunit TRAP suggests a general mechanism

Many critical cellular functions are performed by multisubunit circular protein oligomers whose internal geometry has evolved to meet functional requirements. The subunit number is arguably the most critical parameter of a circular protein assembly, affecting the internal and external diameters of the assembly and often impacting on the protein's function. Although accurate structural information has been obtained for several circular proteins, a lack of accurate information on alternative oligomeric states has prevented engineering such transitions. In this study we used the bacterial transcription regulator TRAP as a model system to investigate the features that define the oligomeric state of a circular protein and to question how the subunit number could be manipulated.We find that while Bacillus subtilis and Bacillus stearothermophilus TRAP form 11-subunit oligomers, the Bacillus halodurans TRAP exclusively forms 12-subunit assemblies. Significantly, the two states of TRAP are related by a simple rigid body rotation of individual subunits around inter-subunit axes. We tested if such a rotation could be induced by insertion or deletion mutations at the subunit interface. Using wild type 11-subunit TRAP, we demonstrate that removal of five C-terminal residues at the outer side of the inter-subunit axis or extension of an amino acid side chain at the opposite, inner side, increased the subunit number from 11 to 12. Our findings are supported by crystal structures of TRAP oligomers and by native mass spectrometry data.The subunit number of the TRAP oligomer can be manipulated by introducing deletion or addition mutations at the subunit interface. An analysis of available and emerging structural data on alternative oligomeric states indicates that the same principles may also apply to the subunit number of other circular assemblies suggesting that the deletion/addition approach could be used generally to engineer transitions between different oligomeric states